Membrane filtration as an effective alternative to manual isolation for soil tardigrade DNA extraction

Résumé fourni par la source

Manual isolation of tardigrades for molecular analysis is time-consuming and limits large-scale biodiversity studies. To enhance the efficiency of soil tardigrade molecular research, we developed a membrane filtration method to enrich soil tardigrades and other microfauna (nematodes and rotifers) for DNA extraction and the subsequent sequencing analyses. Filtration accelerated the workflow and detected more tardigrade species than manual isolation, although all membrane extraction methods underestimate their relative abundance. Filtration with a 15 μm membrane yielded the highest richness, while community differences were mainly driven by soil types rather than extraction methods. Our results highlight the potential of filtration approaches in saving sample processing time and improving the detection of tardigrade species richness.

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