A Single Genomic Region Controls Primocane Fruiting in Tetraploid Blackberry
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Le résumé fourni par la source
Abstract The fresh-market blackberry ( Rubus subgenus Rubus ) industry has expanded dramatically in the past two decades, driven in part by improved cultivars. Introgression of the primocane-fruiting (PF; annual flowering) trait into elite germplasm has enabled dual cropping in a single year, season extension, and cultivation in tropical and subtropical regions. Despite its economic performance, the genetic basis of PF is not well understood. It has been proposed that the PF trait is controlled by a major recessive locus, but its genomic location is unclear. Here, a genome-wide association study (GWAS) of 365 tetraploid blackberry genotypes identified a single genomic region on chromosome Ra03 (∼33 Mb) strongly associated with PF. Genetic linkage analysis in a biparental population confirmed that the same interval (32-35 Mb) was linked to the PF phenotype. Ten putative candidate genes were identified in this region. Allele mining using whole-genome resequencing of 17 genotypes highlighted two high-priority candidates: a CCCH-type zinc finger gene and a ubiquitin-specific protease gene. Use of an improved Rubus argutus ‘Hillquist’ genome annotation (v1.2) enabled refined variant interpretation, including identification of regulatory 3′ UTR polymorphisms in the zinc finger homolog. Two diagnostic KASP markers ( PF1 and PF2 ), designed from the most significant GWAS SNPs, predicted the PF phenotype with over 96% accuracy in a validation panel of 494 tetraploid blackberries from multiple breeding programs. Together, these results provide the first high-resolution mapping of the PF locus in blackberry, identify candidate genes for flowering regulation in Rubus , and deliver diagnostic markers that can be immediately deployed in breeding programs.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A Single Genomic Region Controls Primocane Fruiting in Tetraploid Blackberry
- Date Crossref
- 17/12/2025
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Arkansas at Fayetteville pays non établi dans la noticeUniversité ou école supérieure
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United States Department of Agriculture pays non établi dans la noticeOrganisme public
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Lawrence Berkeley National Laboratory pays non établi dans la noticeStructure de recherche
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North Carolina State University pays non établi dans la noticeUniversité ou école supérieure
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University of Arkansas Fayetteville pays non établi dans la noticeUniversité ou école supérieure
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Hortifrut Genetics pays non établi dans la noticeInstitution
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Lawrence Berkeley National Laboratories pays non établi dans la noticeInstitution
University of Arkansas at Fayetteville, United States Department of Agriculture et Lawrence Berkeley National Laboratory, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.