Genome-Wide Characterization of the YTH Proteins in Salix suchowensis

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YT521-B homology (YTH) domain-containing RNA-binding proteins, the earliest identified and most well-known m6A reader proteins, play important roles in post-transcriptional regulation of plant growth and development as well as stress response by specifically recognizing m6A-modified RNA and subsequently recruiting downstream effector proteins to mediate the biological effects of m6A modification in eukaryotes. In recent years, the identification and functional characterization of YTH family proteins in woody plants have significantly advanced. However, a systematic identification of the YTH proteins has not yet been reported in Salix suchowensis (S. suchowensis), an early-flowering shrub serving as a valuable model for basic genetic research in woody plants. In this study, we identified 11 YTH genes, named SsYTH1-SsYTH11, located on 9 of 19 chromosomes in S. suchowensis. All proteins with a highly conserved YTH domain were classified into 4 distinct subfamilies based on the phylogenetic analysis. The MEME analysis showed that two conserved motifs, motif 1 and motif 2, were distributed in most SsYTH proteins. Promoter cis-acting element analysis of these proteins suggested a potential close association with abiotic stress and hormones. Subsequently, expression analysis following abscisic acid (ABA) and jasmonic acid (JA) treatments demonstrated significant differential expression of several SsYTH genes, thereby establishing a basis for further exploration of the YTH function in S. suchowensis and contributing to the broader understanding of epigenetic regulation in woody plants.

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