OsGLP3-7 negatively regulates drought tolerance by suppressing amino acid, lipid, and jasmonic acid metabolic pathways in rice

Le résumé fourni par la source

• OsGLP3–7 expression is significantly down-regulated by drought stress in rice. • OsGLP3–7 functions as a negative regulator of drought tolerance in rice. • Knockout of OsGLP3–7 inhibits genes involved in JA, amino acid and lipid metabolism. • Knockout of OsGLP3–7 reduces accumulation of JA, amino acid derivatives, and lipids. Although germin-like protein (GLP) family members have been demonstrated to play important roles in regulating plant biotic and abiotic stresses, their specific roles in regulating rice drought stress response are still largely unknown. In this study, we identified that the transcription of OsGLP3–7 was significantly down-regulated by PEG, cold and NaCl stress treatments. Knockout of OsGLP3–7 enhanced plant tolerance whereas overexpression of OsGLP3–7 decreased plant tolerance to PEG6000-mimicked drought stress and water-deficient drought stress, as manifested by the survival rates, water loss rates and ion leakages compared to the wild-type plants. Transcriptomic analysis showed that the expression levels of genes involved in jasmonic acid (JA) metabolism, amino acid metabolism and lipid metabolism were remarkably lower in the OsGLP3–7 knockout plants but higher in the OsGLP3–7 overexpressing plants relative to the wild-type plants under stress treatment. Correspondingly, metabolome analysis revealed that the OsGLP3–7 knockout plants had lower JA, amino acids and lipids contents, whereas the OsGLP3–7 overexpressing plants had higher JA, amino acids and lipids contents than wild-type plants. Exogenous methyl jasmonate (MeJA) treatment reduced the drought tolerance of OsGLP3–7 knockout plants. These findings suggest that OsGLP3–7 negatively regulates rice drought tolerance by inactivating the JA, amino acid and lipids metabolic pathways, holding a promise target for improving drought tolerance through gene editing approaches.

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