Integrated Multi-Omics Reveals Anthocyanin Biosynthesis Control During Fruit Peel Color Development in Jaboticaba ‘Essart’

Le résumé fourni par la source

The color change in the peel of Jaboticaba (Myrciaria cauliflora Berg) ‘Essart’ is primarily driven by the spatiotemporal regulation of anthocyanin biosynthesis, but its molecular mechanism remains unclear. This study employed a multi-omics integrated analysis approach, combining targeted metabolomics, transcriptomics, and small RNA sequencing, to systematically elucidate the regulatory mechanism underlying color change during Jaboticaba fruit peel development. The results showed that during the color-turning stage, the content of most anthocyanins tended to decrease, while the content of Cyanidin significantly increased during the fully ripe stage. Weighted Gene Co-expression Network Analysis (WGCNA) identified the brown module as a highly relevant module for anthocyanin accumulation, which includes a co-expression network of 98 transcription factors and 6 structural genes (F3H, CHI, ANS, CHS). Furthermore, small RNA sequencing results discovered a novel regulatory relationship: plant-MIR408-4—McMYB88. This regulatory relationship exhibited precise temporal dynamics: during the green fruit stage, plant-MIR408-4 was highly expressed and McMYB88 was lowly expressed, thereby inhibiting anthocyanin synthesis; however, during the fully ripe stage, plant-MIR408-4 expression decreased and McMYB88 expression increased, promoting anthocyanin accumulation. In summary, this study revealed the molecular regulatory mechanism of color formation in Jaboticaba fruit peel, providing an important theoretical basis for its color improvement and molecular breeding.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.