A novel vascularized hydrogel by encapsulation of lyophilized platelet-rich fibrin into gelatin methacryloyl hydrogel for bone regeneration

Le résumé fourni par la source

Vascularized osteogenesis is crucial for bone regeneration. Platelet-rich fibrin (PRF) includs many growth factors, of which VEGF plays the significant role in vascularization. No study has investigated the effect of lyophilized platelet-rich fibrin (Ly-PRF) combined with gelatin methacryloyl (GelMA) on the repair of bone defects. We first prepared fresh PRF by one-step centrifugation method. Then, we constructed Ly-PRF by lyophilization. Afterwards, GelMA was synthesized by chemical synthesis method and GelMA hydrogel was prepared by photo-crosslinking method. Finally, Ly-PRF was encapsulated into GelMA hydrogel to construct GelMA@Ly-PRF hydrogel and used in vitro and in vivo to explore its effect on calvarial regeneration of rats. The fresh PRF had good elasticity. GelMA was synthesized by chemical synthesis method and GelMA hydrogel was formed by blue light-irradiation of GelMA solution. After encapsulation of Ly-PRF into GelMA hydrogel, VEGF in Ly-PRF could slowly and sustainably release for continuous 21 days. In vitro, the GelMA@Ly-PRF hydrogel had high swelling property and porous structure. What's more, it had good cytocompatibility and was capable of promoting angiopoiesis of human umbilical vein endothelial cells (HUVECs). In vivo, the GelMA@Ly-PRF hydrogel could facilitate to repair calvarial defects of rats by promoting formation of new blood vessel during the process of bone healing, evidenced by Micro-CT, H&E staining, Masson's staining and immunohistochemical staining of CD31 and OPN. Encapsulation of Ly-PRF into a GelMA hydrogel could promote bone regeneration of rats.

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