Detection and Serotyping of Dengue Virus by Single-Plex Multicolor Real Time RT-qPCR

Le résumé fourni par la source

Dengue remains one of the most significant tropical diseases, with its geographic range continuing to expand. The etiological agent of the disease is dengue virus, a term that groups four closely related viruses defined as serotypes. The four dengue virus serotypes elicit distinct serological responses, and their replacement or co-circulation in endemic regions can result in major outbreaks. The identification of the circulating serotypes is essential for surveillance and clinical management. Here we describe a novel method for the detection and serotyping of the four dengue virus serotypes using a single plex multicolor real-time RT-qPCR. The method takes advantage of a highly informative dataset that includes a realistic representation of globally circulating strains, allowing the selection of a universal primer pair and four serotype-specific hydrolysis probes. The method has been extensively tested and the results support its use in surveillance studies as well as in clinical settings.

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