Hypo-glycosylated FSH enhances the ovarian microenvironment for follicular development compared to fully glycosylated FSH
Rattachement africain : cn, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Differential N-glycosylations of follicle-stimulating hormone (FSH) influence follicular development dynamics, significantly affecting assisted reproductive technology outcomes. However, the ovarian microenvironment induced by different FSH glycoforms remains incompletely understood. In this study, in vivo analyses using RT-qPCR, single-cell RNA sequencing, and bulk RNA sequencing demonstrated that FSH administration rapidly activated responses in both granulosa cells (GCs) and theca cells (TCs) within 2 h. Hypo-glycosylated FSH (hgFSH) initiated GCs and TCs differentiation more effectively than fully glycosylated FSH (fgFSH), and genes with higher expression in hgFSH were predominantly located in these cell types. HgFSH significantly upregulated 20.2% of total ovarian ligand genes, suggesting a more actively ovarian communicating microenvironment. In contrast, fgFSH inhibited the expression of oocyte-specific ligands such as Bmp15, Gdf9 and Oosp1, potentially disrupting oocytes-GCs communication. More importantly, hypo-glycosylated FSH upregulated genes significantly enriched in TCs. HgFSH activated key biological pathways in TCs such as lipid metabolic process and enhanced communication between GCs and TCs through more interactions than fgFSH. Our findings suggest that hgFSH fosters a more effective ovarian microenvironment for follicular development compared to fgFSH, highlighting the potential clinical benefits of hgFSH applications.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Hypo-glycosylated FSH enhances the ovarian microenvironment for follicular development compared to fully glycosylated FSH
- Date Crossref
- 05/12/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Huazhong Agricultural University Frontiers Science Center for Animal Breeding and Sustainable Production pays non établi dans la noticeUniversité ou école supérieure
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Veterans Health Administration pays non établi dans la noticeOrganisme public
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Omaha VA Medical Center pays non établi dans la noticeÉtablissement de santé
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University of Nebraska Medical Center Department of Obstetrics and Gynecology pays non établi dans la noticeÉtablissement de santé
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College of Animal Science and Technology pays non établi dans la noticeUniversité ou école supérieure
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National Center for International Research on Animal Genetics pays non établi dans la noticeInstitution
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Veterans Affairs Medical Center pays non établi dans la noticeÉtablissement de santé
Frontiers Science Center for Animal Breeding and Sustainable Production — Huazhong Agricultural University, Veterans Health Administration et Omaha VA Medical Center, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.