Janus Nanohybrids Enable Superflash Warming and High‐Affinity Ice Confinement for Cross‐Scale Cryopreservation

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Abstract Strong hydration of cryoprotective agents reduces the glass transition temperature of water and suppresses ice formation. However, lethal cooling and warming remain a critical obstacle to cross‐scale cryopreservation of clinical biospecimens. Herein, a snowman‐like Janus nanohybrid composed of magnetic iron tetraoxide and photothermal polypyrrole is reported. Its cranial–corporal asymmetry enables heterogeneous hydration for a record high efficiency of ice confinement, reducing mean ice crystal area by 98.4%. Molecular dynamics simulations reveal that Janus architecture simultaneously enhances interactions with ice and strengthens the local hydration anisotropy, accounting for the effective inhibition of ice growth. Superflash warming over 920 °C min ‒1 by magnetically rotating its anisotropic structure for uniform heat dissipation narrows the hostile temperature window in micro‐/macroscopic scenarios, as further confirmed by Monte Carlo modeling. This design enables cost‐effective post‐thaw magnetic retrieval, eliminating the need for heavy centrifuges, well‐suited for scalable and on‐site applications. As a result, cryopreserved samples from single cells, bacteria, to porcine trachea retain near‐complete viability and functionality. Therefore, this study offers a promising technique to bridge the gap between microscale cell storage and whole‐organ preservation.

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