Comparative Multi-Omics Insights into Flowering-Associated Sucrose Accumulation in Contrasting Sugarcane Cultivars

Le résumé fourni par la source

Flowering often perturbs carbon allocation in sugarcane, yet its transcriptomic–metabolomic basis remains unclear. We profiled two contrasting cultivars, Gui Tang 16-3285 (sugar increases during flowering) and Gui Tang 44 (sugar decreases), sampling apical tissues at five stages (Non-spikelet-bearing stage (NSB), Early booting stage (ESB), Late booting stage (LSB), Tasseling stage (TS), and Flowering stage (FS)). RNA-seq and untargeted LC–MS revealed a strong stage/genotype structure (PCA) with high reproducibility. Pairwise contrasts (FS vs. earlier stages) and time series clustering (Mfuzz) showed extensive, stage-resolved reprogramming with small cross-cultivar overlaps. GO/KEGG indicated that GT16 is enriched for central carbon processes and glucose response, whereas GT44 favors cell-wall remodeling (xylan/xyloglucan), amino/nucleotide sugar, and phenylpropanoid pathways. Integrated analysis identified opposing temporal features across omics layers: in GT16, late-rising metabolites—including sedoheptulose—were consistent with enhanced pentose phosphate/Calvin coupling that regenerates fructose-6-phosphate for sucrose biosynthesis; in GT44, early activation of wall and secondary sinks, together with trehalose/(trehalose-6-phosphate) T6P signatures, paralleled declining soluble sugars. Across cultivars we resolved 11 and 18 genes in reciprocal opposite-trend sets (most with clear temporal order) and eight vs. five metabolites with mirrored dynamics, nominating actionable biomarkers (e.g., sedoheptulose/S7P) and regulatory nodes. These results provide a mechanistic framework linking flowering stage to carbon partitioning and suggest practical levers—timing growth moderation/ripeners, prioritizing sucrose phosphate synthase/Sucrose Phosphate Phosphatase, tempering wall flux, to sustain sucrose during reproductive development and inform breeding for high-sugar, flowering-resilient ideotypes.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.