Supplementary Tables and Figures from Triple-Negative Breast Cancer-Derived Extracellular Vesicles Promote a Hepatic Premetastatic Niche via a Cascade of Microenvironment Remodeling

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Supplementary Table 1. Information about patients derived xenograft models and derived patient.Supplementary Table 2. Ontology of differentially expressed genes in metastasis-harboring liver of PDX models.Supplementary Table 3. Differentially expressed genes in liver and lung of metastasis-harboring PDX models.Supplementary Table 4. Human ratio in lung and liver of additional PDX models identified by RNA sequencing.Supplementary Table 5. Gene ontology of co-expressed genes with Cx3cr1 in liver of PDX models identified by RNA sequencing.Supplementary Table 6. Top ranked genes having significant correlation with Cx3cr1 in liver of PDX models identified by RNA sequencing.Supplementary Table 7. Demographic and clinical characteristics of all patients.Supplementary Table 8. Information about used primer sequences for quantitative real-time PCRSupplementary Figure 1. Liver and lung H&E staining pictures of eight nonmetastatic PDX models a. Microscopic images of non-metastasis harboring PDX mouse model's liver (left) and lung (right) taken at 400x magnification.Supplementary Figure 2. Immunohistochemistry about CX3CR1 in liver and lung of breast cancer metastasis patients.Supplementary Figure 3. Hepatic metastasis after four weeks after orthotopic injection of 4T1 breast cancer cells identified by H&E staining.Supplementary Figure 4. Identification of pre-metastatic niche in liver tissues of 4T1 bearing mice a. mRNA expression of inflammation associated genes identified by real-time PCR in liver tissues of 4T1 bearing mice.Supplementary Figure 5. Identification of CX3CR1 positive cell population.Supplementary Figure 6. FACS analysis with anti-CX3CR1 of Raw264.7 macrophage cell lines.Supplementary Figure 7. Trans-well migration assay of BMDM with or without CX3CL1.Supplementary Figure 8. Mmp9 mRNA expression having significant correlation with Cx3cr1 mRNA expression in liver of PDX models.Supplementary Figure 9. MMP9 expression of macrophages with CX3CL1 treatment.Supplementary Figure 10. Signaling pathway of CX3CL1 treated macrophages.Supplementary Figure 11. Splenic injection of 4T1 cells inducing hepatic metastasis within two weeks in most cases.Supplementary Figure 12. mRNA expression about Cx3cr1 and Mmp9 in liver of vehicle or AZD8797 treated BALB/C mice identified by real-time PCR.Supplementary Figure 13. Correlation plot of Tnfa and Cx3cr1 mRNA expression in liver and lung of PDX models.Supplementary Figure 14. EVs isolation of CD63-mRuby-4T1.

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