Toxicity evaluation of laser-synthesized pro-angiogenic carbon monoxide-rich gold nanoparticles in vitro and in vivo
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Le résumé fourni par la source
Introduction The safety profile of gold nanoparticles remains a concern and depends on dose, size, surface chemistry and charge. This study evaluated through in vitro and in vivo methods the cytotoxicity, oxidative stress and genotoxicity of laser synthesized carbon monoxide-Rich Gold Nanoparticles (COR-AuNPs), which can strongly promote angiogenesis in endothelial colony forming cells (ECFC). Methods COR-AuNPs were synthesized by pulsed laser driven CO 2 reduction reaction, and stabilized for culture media with the addition of the copolymer Pluronic-F127 (PF127). The fresh synthetized nanoparticles where characterized for size and morphology. Their stability was investigated in both culture media and the water aquarium environment. In vitro Cytotoxicity was assessed using the MTS assay on SaOS-2 cells and trypan blue staining in ECFCs at concentrations of 5 and 10 × 10 3 μg.L −1 . Zebrafish were exposed to a maximum concentration of 75 μg.L −1 of COR-AuNPs for 96 h. Oxidative stress biomarkers were assessed in liver and brain tissues, while genotoxicity was evaluated using the comet assay. The analyzed biomarkers included superoxide dismutase, catalase, reduced glutathione, total antioxidant capacity, carbonylated protein, and malondialdehyde. Results The PF127 stabilized COR-AuNPs are stable in the zebrafish aquarium water for 6 weeks and showed no precipitation in the culture media. A strong pro-angiogenic activity was observed for ECFCs exposed to the COR-AuNPs at a minimal concentration of 5 × 10 3 μg.L −1 . The CO release is not immediate in the culture medium, suggesting that the COR-AuNPs are characterized by an intracellular release. No significant cytotoxicity was observed in both ECFC or SaOS-2 cells, and most oxidative stress biomarkers showed no significant effects in zebrafish. However, reduced glutathione levels decreased significantly in the brain at concentrations between 10 and 35 μg.L −1 , likely due to the interaction with the metallic surface of the nanoparticles, while in the liver they increased significantly following exposure to COR-AuNPs at concentrations between 20 and 75 μg.L −1 . No genotoxic effects were detected in zebrafish. Conclusion COR-AuNPs enhance capillary morphogenesis in ECFCs, with minimal cytotoxicity, oxidative stress, and genotoxicity at sublethal concentration, supporting their safety for potential applications in regenerative therapies.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Toxicity evaluation of laser-synthesized pro-angiogenic carbon monoxide-rich gold nanoparticles in vitro and in vivo
- Date Crossref
- 26/11/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Escola Nacional de Saúde Pública pays non établi dans la noticeUniversité ou école supérieure
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Fundação Oswaldo Cruz pays non établi dans la noticeStructure de recherche
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Pontifícia Universidade Católica do Rio de Janeiro pays non établi dans la noticeUniversité ou école supérieure
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Instituto Nacional de Metrologia pays non établi dans la noticeOrganisme public
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Universidade Federal do Rio de Janeiro pays non établi dans la noticeUniversité ou école supérieure
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Universidade Federal do Estado do Rio de Janeiro pays non établi dans la noticeUniversité ou école supérieure
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University of Florence Department of Experimental and Clinical Biomedical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Laboratory for Environmental Health Assessment and Promotion pays non établi dans la noticeStructure de recherche
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National School of Public Health Postgraduate Program in Public Health and the Environment pays non établi dans la noticeUniversité ou école supérieure
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Pontifical Catholic University of Rio de Janeiro Laboratory of Laser Synthesis and Characterization of Nanomaterials pays non établi dans la noticeUniversité ou école supérieure
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National Institute of Metrology Postgraduate Program in Biotechnology pays non établi dans la noticeStructure de recherche
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Federal University of the State of Rio de Janeiro Department of Natural Sciences pays non établi dans la noticeUniversité ou école supérieure
Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz et Pontifícia Universidade Católica do Rio de Janeiro, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.