Late Breaking Abstract - Single-cell analysis identifies rare CCLHi monocyte-derived alveolar macrophages as potential ILD biomarker
Rattachement africain : gr, de, Égypte. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Interstitial lung disease (ILD) represents a heterogeneous group of lung disorders whose cellular and molecular mechanisms are incompletely understood. This clinical study aimed to elucidate underlying immune mechanisms at single-cell resolution focusing on alveolar macrophages and the periphery. A total of 44 fibrotic ILD patients (9 IPF, 11 CHP, 7 RA-ILD, 5 SSc-ILD, 12 unclassifiable ILD) and 8 non-ILD controls were recruited at a single medical center. Bronchoalveolar lavage (BAL) and peripheral blood were collected the same day and single-cell RNA-sequencing was performed via the BD Rhapsody system. Lung function assessments were performed according to pan-European guidelines. Molecular BAL (205,392 cells) and peripheral blood (312,727 cells) cell atlases revealed substantial alterations of the global BAL cell population including a significant expansion of monocyte-derived alveolar macrophage (MoAM) states in ILD. In addition to previously described SPP1Hi MoAM, we identified a rare CCLHi MoAM state (~2% of BAL cells) that was transcriptionally linked to inflammatory and fibrosis-associated processes and identified as a key signaling node in the ILD lung. Moreover, the proportion of CCLHi MoAM inversely correlated with lung function as determined by DLCOpp (R=-0.4). The MoAM increase was also reflected by a significant increase of classical monocytes (CMo) in the periphery of ILD patients. In summary, this study identifies a rare, inflammation and fibrosis associated CCLHi MoAM population that expanded across ILD subtypes and is linked to disease severity, supporting its role in ILD pathogenesis and as a potential therapeutic target and biomarker.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Late Breaking Abstract - Single-cell analysis identifies rare CCLHi monocyte-derived alveolar macrophages as potential ILD biomarker
- Date Crossref
- 27/09/2025
- Éditeur
- European Respiratory Society
- Type
- proceedings-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Crete pays non établi dans la noticeUniversité ou école supérieure
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German Center for Neurodegenerative Diseases pays non établi dans la noticeStructure de recherche
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Boehringer Ingelheim (Germany) pays non établi dans la noticeEntreprise
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Boehringer Ingelheim (Egypt) Égypte (code pays fourni par la source)Entreprise
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School of Medicine Laboratory of Molecular and Cellular Pneumonology pays non établi dans la noticeUniversité ou école supérieure
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University of Bonn and West German Genome Center German Center for Neurodegenerative Diseases (DZNE) pays non établi dans la noticeUniversité ou école supérieure
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Boehringer Ingelheim International GmbH pays non établi dans la noticeEntreprise
University of Crete, German Center for Neurodegenerative Diseases et Boehringer Ingelheim (Germany), avec 4 autres affiliations. Pays d’affiliation : Égypte.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.