Bone marrow Mesenchymal Stromal Cells stimulated with Pulsed Electromagnetic Fields for the treatment of pulmonary fibrosis

Résumé fourni par la source

Mesenchymal stromal cell (MSCs) role in regulating inflammation, angiogenesis, and extracellular matrix remodeling was previously demonstrated. Our results on Chronic Lung Allograft Dysfunction patients showed FEV1 stabilization and no adverse events attributable to the Bone Marrow (BM)-MSCs infusion. The beneficial effects of MSCs-based treatment are mediated by the release of paracrine factors, extracellular vesicles, and mitochondrial transfer, which contribute to the resolution of endo-alveolar inflammation and fibrotic progression. These processes result in anti-inflammatory and pro-resolving effects on injured lung endothelium and alveolar epithelium. Various pre-conditioning protocols are reported to enhance the therapeutic effect of MSCs and many works have shown how pulsed electromagnetic fields (PEMFs) can influence the biological processes of MSCs. Cell proliferation (cumulative population doubling), surface antigen expression (flow cytometry), immunomodulation capacity, senescence and karyotype stability of healthy donors' BM-MSCs cultured under PEMFs were compared to untreated MSCs. Additionally, reactive oxygen species (ROS) generation were assessed, given the detrimental role of excessive ROS in some clinical setting such as wound healing. Our results showed a reduced ROS production by PEMF-MSCs compared to untreated MSCs, while comparable immunomodulatory properties, cell antigen surface expression, and genomic stability were observed. These findings highlight the PEMF potential as BM-MSC conditioning to mitigate oxidative stress and accelerate the transition beyond the inflammatory phase.

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