Figure 3 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia

Résumé fourni par la source

Effect of NKCEs targeting CD19 or CD20 on primary BCP-ALL cells. A, Phenotypic characterization via flow cytometry of four BCP-ALL primary leukemia samples, using CD45-APC-Vio770, CD19-PE-Cy7, and CD20-V450. B, 7AAD/AnnV staining of cells from sample ALL#06 cultured either alone (only ALL#06) or with resting NK cells from a representative healthy donor and the indicated NKCEs (100 μg/mL). C, CD107a expression of NK cells cultured either alone or with cells from ALL#06 in the presence of IC-NKp46-NKCE, CD19-NKp46-NKCE, or CD20-NKp46-NKCE (100 μg/mL). A representative experiment is shown. Numbers indicate the percentage of cells in each quadrant. D, Percent of specific lysis of CD19+ leukemia blasts (left) and CD107a degranulation (right) of resting NK cells from healthy donors (n = 3–6) upon coculture with primary leukemia blasts and NKCEs at 100 μg/mL as indicated. Data obtained with target cells containing leukemia blasts <85% (ALL#02 and ALL#07) or >85% (ALL#04 and ALL#06) were pooled. Results from 2–5 independent experiments are reported. The incubation time for all the tests was 4 hours. Bar show mean ± SEM. Statistical significance: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. Mann–Whitney test was used to calculate statistical differences.

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