Figure S2 from Molecularly Defined Subsets of Ewing Sarcoma Tumors Differ in Their Responses to IGF1R and WEE1 Inhibition

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Cell cycle analyses A. Dansylcadaverine-mediated nIGF1R depletion arrested CCH1 cells at the G1-phase and reduced the percentage of cells in the S-phase. B. Western blots show that Cyclin A2 and Cyclin D1 levels were high in CCH5 cells with nIGF1R relative to NCH6 with mIGF1R. C. Cell cycle analyses shows higher percentages of CCH1 and CCH5 cells in S-phase than CCH2 and NCH6 cells. D. CCH5 cells with nIGF1R divided faster, had higher percentages of S-phase cells with shorter S-phase duration than NCH6 with mIGF1R. E. EdU/BrdU double labelling-based S-phase duration analysis was carried out. Top panel shows that cells showed EdU-AF55+ and BrdU-FITC+ signals in comparison to unstained cells. F. Percentages of EdU+BrdU+ cells (CCH1, CCH5, CCH2 and NCH6 cells), at indicated time points, are shown. G.Timings of S-phase exit (red) for all cells were calculated using linear regression.

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