Figure 3 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival

Résumé fourni par la source

ATF4 is required in DLBCL cells and is partially responsible for SIRT3′s functions to promote DLBCL cell proliferation and survival. A, Effect of ATF4 knockdown on the proliferation of DLBCL cell lines. Each cell line was infected with lentivirus expressing control or ATF4 shRNAs and yellow fluorescent protein (YFP), and YFP+ viable (DAPI−) cells were monitored by flow cytometry for 8 days. B, FCs of cell numbers of HBL1 cells expressing control or SIRT3 shRNA with or without exogeneous ATF4. HBL1 cells were transduced with viral vectors containing shRNAs or genes as presented. Cell number changes were normalized to data of initial time point (day 3 after infection). C, Summarized results show the rescue effects of exogenous ATF4 to SIRT3 shRNA-induced cell proliferation inhibition in different DLBCL cells. The data were summarized from day 10 after infections and normalized to the cell numbers of their respective control shRNA–expressing cell. D, Effects of exogenous ATF4 on different DLBCL cells expressing control or SIRT3 shRNAs. Dead cells were stained with DAPI and quantified through flow cytometry. The relative cell death was calculated by normalizing the percentage of dead cells in control shRNA–expressing cells in respective cell lines. *, P < 0.05; **, P < 0.01. Error bars represent the mean ± SD of three or more replicates.

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