Figure 1 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival

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Knocking down SIRT3 caused ATF4 signaling inhibition but not HIF1a. A, Dendrograms from hierarchical clustering of RNA-seq data from three DLBCL cells lines transduced with lentiviruses containing control (scramble) or two SIRT3 shRNAs. B, Heatmap showing differential expression in SIRT3 knockdown cells versus control (FC > 1.5, q < 0.05). C, Heatmap showing enrichment of SIRT3 knockdown signatures within key pathways. CHIP, chromatin immunoprecipitation; CHOP, C/EBP homologous protein; dn, down; KEGG, Kyoto Encyclopedia of Genes and Genomes; MEF, mouse embryonic fibroblast; TM, tunicamycin. D, GSEA (51, 52) showing the enrichment of ATF4 target genes in SIRT3-downregulated genes in Karpas 422, OCI-LY1, and HBL1 cells with SIRT3 sh1 versus control scramble shRNAs. The rank lists were from RNA-seq analysis from B. ATF4 target genes were summarized from previous publications (17, 18). E, GSEA (51, 52) showing the enrichment of ATF4 target genes in SIRT3-downregulated genes in Karpas 422, OCI-LY1, and HBL1 cells with SIRT3 sh2 versus control scramble shRNAs. The rank lists were from RNA-seq analysis from B. The same ATF4 target gene list was used here as in D.

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