Hunting of Endogenous and Exogenous Radical Species into DNA Cleavage Catalysed by a Mn2+-dependent Deoxyribozyme

Le résumé fourni par la source

DNAzymes, catalytically active DNA molecules, do perform DNA cleavage through metal ion co-factors (dia- and para-magnetic cofactors). The radical path through which the cleavage occurs is characterized by several radical species. Identify those species, as well as the fine tuning of the cofactors’ activities is a key role into the realm of such in vitro selected DNA-based enzymes. In this work, different radical species were identified, both for the cleavage reaction and for the stabilization of the oligonucleotides’ architecture. The integrative approach proposed here has a particular focus on the high-spin metal co-factor(s). An EPR multifrequency approach was able to deliver consistently and robust outcomes of the spectroscopic parameters, validating contributions affecting the nature of the radical species. Despite the super-stoichiometric ratio (Metal/DNAzyme) and the presence of several different salts used to stabilize the global architecture, EPR study was able to decipher paramagnetic species generated during the different steps of the cleavage reaction.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.