Prussian blue nanoparticles-based dual-mode immunochromatographic assays for norfloxacin detection in animal-derived foods

Résumé fourni par la source

Prussian Blue Nanoparticles (PBNPs)-based dual-mode immunochromatographic assays (ICAs) were developed for the detection of norfloxacin (NOR) in animal-derived foods. The assays used the inherent bright blue color and peroxidase-like catalytic activity of PBNPs. The visual limit of detection (vLOD) of the developed ICAs were both determined to be 1 ng mL −1 . The calculated limit of detection (cLOD) of the ICA based on the inherent bright blue color of PBNPs was 0.5 ng mL −1 , while that of the ICA using the peroxidase-like catalytic activity of PBNPs was 0.34 ng mL −1 . Analysis of spiked samples demonstrated that NOR could be effectively detected in pork, chicken, beef, sea bass and shrimp, with recoveries ranging from 80.43 % to 106.33 %. The results showed strong correlation with those obtained by HPLC, with R 2 values of 0.9653 and 0.9715, respectively. Therefore, this study established a sensitive, accurate, rapid and cost-effective method for detecting NOR in animal-derived foods. • PBNPs-based dual-mode immunochromatographic assays were developed to detection NOR. • The developed ICAs showed advantages of high sensitivity and rapidity. • The accuracy of the developed ICAs for detection NOR was verified by HPLC.

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