Peer Review Report For: Association between intestinal bacterial carriage, biomarkers of environmental enteric dysfunction, and stunting in rural Malawian children [version 1; peer review: 1 approved with reservations, 1 not approved]
Rattachement africain : Malawi, gb, kh. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Background . Available data from murine studies suggest that intestinal bacteria may have a role in modulating growth phenotypes in the host. We investigated the prevalence of four gut bacteria known in murine models to impair growth ( Bifidobacterium longum, Faecalibacterium prausnitzii, Dorea formicigenerans, and Akkermansia muciniphila ), the level of fecal biomarkers of environmental enteric dysfunction (EED) and stunting in rural Malawian children. Methods. DNA and protein were extracted from fecal samples of rural Malawian children (aged 1-59 months) at a baseline cross-sectional survey in the Mangochi district of Malawi conducted within the framework of the Macrolides Oraux pour Réduire les Décès avec un Oeil sur la Résistance (MORDOR) trial. Intestinal carriage of bacteria was measured by PCR. Neopterin (NEO), myeloperoxidase (MPO), and alpha-1 antitrypsin (AAT), biomarkers of EED, were measured by an enzyme-linked immunosorbent assay (ELISA) test. Height-for-age Z (HAZ) score <-2 defined stunting. Tests of proportions and regression models were used to explore the relationship between bacterial carriage, EED, and stunting. Results . Fecal samples from 613 children were available for laboratory analyses. F. prausnitzii and D. formicigenerans were prevalent in over 70% of children while B. longum was the least prevalent. B. longum carriage in younger children was associated with elevated EED biomarkers. Two thirds of children had elevated NEO, 33% had elevated MPO, and 16% had elevated AAT. Stunting was found in 38%. No significant associations were found between EED biomarkers or intestinal bacteria carriage and stunting. Conclusion. Intestinal carriage of these four bacteria was not associated with stunting in Malawian children. Carriage was also not associated with EED, nor EED biomarker levels associated with stunting. Further factors acting in concert are necessary to impact EED, perturb growth, and alter gut bacterial carriage.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Peer Review Report For: Association between intestinal bacterial carriage, biomarkers of environmental enteric dysfunction, and stunting in rural Malawian children [version 1; peer review: 1 approved with reservations, 1 not approved]
- Date Crossref
- 25/10/2022
- Éditeur
- F1000 Research Ltd
- Type
- peer-review
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Kamuzu Central Hospital Malawi (code pays fourni par la source)Établissement de santé
-
London School of Hygiene & Tropical Medicine Clinical Research Department pays non établi dans la noticeUniversité ou école supérieure
-
University of Health Science Department of Pathology Blantyre, Malawi (ville ou établissement reconnu, pays non nommé)Université ou école supérieure
-
Kamuzu University of Health Sciences Malawi (code pays fourni par la source)Université ou école supérieure
-
Blantyre Institute for Community Ophthalmology Blantyre, Malawi (code pays fourni par la source)Organisation à but non lucratif
-
School of Public Health and Family Medicine Blantyre, Malawi (ville ou établissement reconnu, pays non nommé)Université ou école supérieure
-
Blantyre Institute of Community Outreach Blantyre Institute of Community Outreach, Malawi (ville ou établissement reconnu, pays non nommé)Structure de recherche
Kamuzu Central Hospital (Malawi), Clinical Research Department — London School of Hygiene & Tropical Medicine et Department of Pathology — University of Health Science (Blantyre, Malawi), avec 4 autres affiliations. Pays d’affiliation : Malawi.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.