Continuous light enhances adventitious rooting in Robinia pseudoacacia L. through a regulatory module involving RpCOP1 and auxin signaling
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Le résumé fourni par la source
Continuous light is a crucial environmental signal, yet its precise role in adventitious rooting in woody plants, particularly concerning the function of the classical light-signaling repressor CONSTITUTIVE PHOTOMORPHOGENIC 1, remains a significant open question. This study investigated how continuous light regulates adventitious root formation in Robinia pseudoacacia L. (black locust) through RpCOP1-mediated mechanisms. Black locust tissue culture plantlets were analyzed through anatomical observation, physiological measurements, hormone profiling, transcriptome sequencing with time-ordered gene co-expression network analysis, and heterologous overexpression validation in poplar. Continuous light treatment advanced rooting by 2 days, increasing adventitious root number by 86.03 % and root length by 110.76 %. The pro-adventitious rooting effect was associated with physiological reprogramming, wherein continuous light reduced photosynthetic efficiency while promoting soluble sugar accumulation at the stem base. Time-ordered gene co-expression network analysis identified RpCOP1 as a key upstream hub whose expression is induced by continuous light. Upregulation of RpCOP1 coincided with systemic hormonal reprogramming, highlighted by 60.72 % increase in auxin. Functional validation via heterologous overexpression of RpCOP1 in poplar supported this positive regulatory role. Compared with wild-type, transgenic lines under continuous light exhibited 33.33 % increase in adventitious root number and 75.85 % increase in root length, demonstrating a photoperiod-dependent effect, with rooting performance ordering continuous light > normal light > continuous dark. These findings support a context-dependent model where RpCOP1 function exhibits either repressive or activating roles depending on light environment and tissue type.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Continuous light enhances adventitious rooting in Robinia pseudoacacia L. through a regulatory module involving RpCOP1 and auxin signaling
- Date Crossref
- 01/12/2025
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Beijing Forestry University pays non établi dans la noticeUniversité ou école supérieure
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Hebei Normal University of Science and Technology pays non établi dans la noticeUniversité ou école supérieure
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University of British Columbia pays non établi dans la noticeUniversité ou école supérieure
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Hebei Normal University of Science & Technology pays non établi dans la noticeUniversité ou école supérieure
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College of Biological Sciences and Technology State Key Laboratory of Tree Genetics and Breeding pays non établi dans la noticeUniversité ou école supérieure
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School of Soil and Water Conservation pays non établi dans la noticeUniversité ou école supérieure
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Anhui Zhongmiao Forestry Development Co. pays non établi dans la noticeOrganisation à but non lucratif
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Beijing Daxing Fruit and Forest Institute pays non établi dans la noticeStructure de recherche
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Department of Forest and Conservation Sciences Faculty of Forestry pays non établi dans la noticeUniversité ou école supérieure
Beijing Forestry University, Hebei Normal University of Science and Technology et University of British Columbia, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.