[Mild moxibustion attenuates vascular and renal aging in senescent rats by regulating the miR-34a/sirt1/p53 signaling pathway].

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OBJECTIVES: To observe the effect of mild moxibustion on microRNA-34a (miR-34a)/silent information regulator 1 (sirt1)/tumor protein p53 (p53) signaling pathway in the thoracic aorta and kidney of senescent rats, so as to investigate the mechanism of mild moxibustion in attenuating vascular and renal aging. METHODS: ) for 4 weeks to establish aging model. Twenty-four successfully modeled rats were randomly divided into aged control, medication and mild moxibustion groups (with 8 rats in each group), with 8 additional 3-month-old male SD rats as the young control group. Rats of the mild moxibustion group received mild moxibustion at "Guanyuan" (CV4) and bilateral "Shenshu" (BL23) once daily for 5 d/week over 8 weeks. Rats of the medication group were intraperitoneally injected with testosterone propionate (7 mg/kg) every 3 d for 8 weeks. The aged and young control groups received equivalent volumes of 0.9% normal saline. Exhaustive swimming time was measured pre- and post-treatment. Histomorphology and collagen content of the thoracic aorta and kidney were assessed using HE and Masson staining. Serum total testosterone (TT) and free testosterone (FT) contents were detected by ELISA. Klotho protein expression in aortic and renal tissue was detected via immunohistochemistry. The relative protein expressions of sirt1, p53, and transforming growth factor-β1 (TGF-β1) were detected using Western blot, while real-time PCR was used to detect miR-34a, sirt1, and p53 mRNA expressions. RESULTS: <0.05). HE staining showed thickened thoracic aorta and reduced glomeruli number in the aged control group, which was improved in the medication and mild moxibustion groups, including thinner thoracic aortic intima, more complete glomerular structure, and more regular renal icles and glomerular lumen, and the improvement in the mild moxibustion group was better than that in the medication group. CONCLUSIONS: Mild moxibustion exerts anti-aging effects on the thoracic aorta and kidney of senescent rats, which may be related with its effect on modulating the miR-34a/sirt1/p53 signaling pathway.

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