Regulation of Circadian Rhythm Disruption in Mice via Transcranial Low-Intensity Focused Ultrasound Stimulation Targeting the Suprachiasmatic Nucleus
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Le résumé fourni par la source
Circadian rhythm disruption (CRD) is a critical contributor to sleep, metabolic, and neuropsychiatric disorders. Ultrasound (US) stimulation represents a non-invasive neuromodulatory modality, yet its impact on CRD remains unexplored. This study is the first to propose and systematically assess the effects of transcranial low-intensity focused ultrasound (LIFU) stimulation targeting the suprachiasmatic nucleus (SCN) in a chronic jet lag mouse model. Circadian and anxiety-related behaviors were evaluated through spontaneous locomotor activity monitoring and open field testing. Plasma melatonin and cortisol levels were quantified via ELISA, and neuronal activity in the SCN, dorsomedial hypothalamus (DMH), and subparaventricular zone (SPZ) was assessed using c-Fos immunofluorescence. Results revealed that US stimulation markedly reduced the phase difference in the US-SCN group to -2.6 h, in contrast to -7.15 h in the CJL group, indicating enhanced circadian alignment with the normal control group in the US-SCN. In the US-SCN group, melatonin and cortisol levels significantly decreased from 214.70 $~\pm ~$ 3.54 pg/mL and 217.21 $~\pm ~$ 5.62 pg/mL to 172.45 $~\pm ~$ 10.16 pg/mL (p = 0.001) and 187.35 $~\pm ~$ 2.57 pg/mL (p = 0.002), respectively. Additionally, anxiety-like behaviors were alleviated in the US-SCN group, as evidenced by a 61.40% increase in the time spent exploring the center zone. Neuronal activity in the SCN, DMH, and SPZ was significantly enhanced. Histological analysis confirmed no tissue damage following LIFU stimulation, demonstrating its safety. LIFU stimulation of the SCN effectively alleviates CRD. This non-invasive approach offers a promising therapeutic strategy for CRD and advancing neuromodulation-based chronotherapy.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Regulation of Circadian Rhythm Disruption in Mice via Transcranial Low-Intensity Focused Ultrasound Stimulation Targeting the Suprachiasmatic Nucleus
- Date Crossref
- 01/01/2025
- Éditeur
- Institute of Electrical and Electronics Engineers (IEEE)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Xi'an Jiaotong University Department of Neurosurgery pays non établi dans la noticeUniversité ou école supérieure
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Sichuan University NHC Key Laboratory of Chronobiology pays non établi dans la noticeUniversité ou école supérieure
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Shanghai Sixth People's Hospital pays non établi dans la noticeÉtablissement de santé
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Second Affiliated Hospital of Xi'an Jiaotong University pays non établi dans la noticeÉtablissement de santé
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and the School of Life Science and Technology Department of Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Shanghai Jiao Tong University School of Medicine Department of Cardiology pays non établi dans la noticeUniversité ou école supérieure
Department of Neurosurgery — Xi'an Jiaotong University, NHC Key Laboratory of Chronobiology — Sichuan University et Shanghai Sixth People's Hospital, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.