A spatial atlas of human gastrointestinal acute GVHD reveals epithelial and immune dynamics underlying disease pathophysiology

Le résumé fourni par la source

Acute graft-versus-host disease (aGVHD) is a severe complication of allogeneic hematopoietic stem cell transplantation, driven by alloreactive donor T cells in the gut. However, the roles of additional donor and host cells in this process are not fully understood. We conducted multiplexed imaging to generate comprehensive spatial maps of healthy and GVHD-afflicted duodena at single-cell resolution. We profiled 59 biopsies from patients with varying gastrointestinal (GI) aGVHD manifestations and 18 healthy controls and measured the spatial expression of 40 proteins, designated to delineate distinct epithelial cell populations of the gut, stromal cells, and immune cells. Normal duodenum was stereotypical across individuals, showing organization and zonation of epithelial, stromal, and immune cells. Patients with aGVHD exhibited increased fibrosis, alterations in crypt morphology, loss of Paneth cells, and accumulation of endocrine cells in the crypts. Homeostatic immune organization was disrupted, with a prominent reduction in immunoglobulin A-secreting plasma cells. CD8 T cells were enriched only in a subset of patients, whereas others exhibited noncanonical enrichments of macrophages and neutrophils. Immune composition was correlated with the time after transplantation, with shared dynamics observed across individuals. Host cells dominated the plasma and T cell compartments in the gut for extended periods of time after transplantation, suggesting that additional players may drive aGVHD across individuals in addition to donor-derived T cells. This spatial atlas of healthy and GI aGVHD uncovers epithelial and immune dynamics across individuals, offering insights into disease pathophysiology and potential clinical applications.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.