Determining Irradiation Dose in Potato Tubers During Storage Using Reaction-Based Pattern Recognition Method

Le résumé fourni par la source

Food irradiation is gaining popularity worldwide as a method for extending shelf life and controlling pests and diseases. Post-treatment irradiation doses are usually monitored using instrumental methods, which may be expensive, labor-intensive, time-consuming, and not allow for low-dose detection. We previously proposed a chemical fingerprinting strategy for estimating irradiation doses based on measuring the rate of an indicator reaction. However, the feasibility of dose assessment was demonstrated only for freshly irradiated samples. In this study, we investigated the feasibility of determining the order of magnitude of dose in irradiated raw potato tubers after several days of storage. The samples were extracted with water, and the extracts were introduced into oxidation-reduction and aggregation reactions carried out in a 96-well plate. The reaction rates were monitored by measuring absorbance and fluorescence of the reaction products, followed by chemo-metric processing. The feasibility to estimate doses to an order of magnitude (0, 100, 1000 Gy) was shown for storage time of 0, 2, and 6 days at 4°C. The accuracy of dose recognition on day 6 was at least 97% by using SoftMax regression (SR) or linear discriminant analysis (LDA). Irradiated and non-irradiated samples can be confidently distinguished using partial least square–discriminant analysis (PLS-DA). The reaction-based method of dose assessment is simple, rapid, and does not require sophisticated equipment.

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