Impact of UV Aging on the Toxicity and Bioavailability of Inductively Coupled Plasma Mass Spectrometry (ICP-MS)-Traceable Core–Shell Polystyrene Nanoplastics in an In Vitro Triculture Small Intestinal Epithelium Model
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Le résumé fourni par la source
A major bottleneck in evaluating the environmental health implications of micro-nanoplastics (MNPs) is the inadequacy of analytical techniques for their precise quantification within complex environmental and biological matrices. Additionally, there is a conspicuous paucity of studies addressing environmentally relevant, photo-aged MNPs. In this study, the effects of UV aging on toxicity and bioavailability were investigated utilizing inductively coupled plasma mass spectrometry (ICP-MS)-traceable 25 nm gold-core polystyrene shell nanoplastics (AuPS25 NPs) and a triculture small intestinal epithelium (SIE) model coupled with simulated digestions to mimic physiological bio-transformations post-ingestion. Employing dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS), the physicochemical and morphological alterations of AuPS25 NPs as a function of UV exposure time were investigated, revealing significant photo-oxidation within 14 days. Toxicological evaluations demonstrated that, contrasting with un-aged AuPS25 NPs, the digesta from UV-aged AuPS25 NPs at oral concentrations of 4 and 40 µg/mL weakened barrier integrity by ~15% and ~18% and heightened cytotoxicity by ~4.3% and ~5.4%, respectively. Although the NP translocation rates were similar for both aged and un-aged PS NPs, the uptake by SIE of aged AuPS25 NPs was significantly higher, reaching 72.2% at 4 µg/mL and 59.2% at 40 µg/mL. In contrast, less than 0.5% of the un-aged PS NPs at both 4 µg/mL and 40 µg/mL were taken up by SIE. These findings highlight the imperative to integrate environmentally aged MNPs into toxicological assessments, as they facilitate "real-world" MNPs. Finally, the use of ICP-MS-traceable core-shell MNPs enables the identification and quantification of PS MNPs in cell lysates and biological media via ICP-MS, showcasing the use of such a tracer MNP approach in cellular uptake and in vivo biokinetic studies.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Impact of UV Aging on the Toxicity and Bioavailability of Inductively Coupled Plasma Mass Spectrometry (ICP-MS)-Traceable Core–Shell Polystyrene Nanoplastics in an In Vitro Triculture Small Intestinal Epithelium Model
- Date Crossref
- 30/10/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Environmental and Occupational Health Sciences Institute pays non établi dans la noticeUniversité ou école supérieure
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Connecticut Agricultural Experiment Station pays non établi dans la noticeOrganisme public
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The State University of New Jersey Rutgers pays non établi dans la noticeUniversité ou école supérieure
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Tulane University pays non établi dans la noticeUniversité ou école supérieure
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Rutgers University Environmental and Occupational Health Sciences Institute (EOHSI) pays non établi dans la noticeUniversité ou école supérieure
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Department of Analytical Chemistry pays non établi dans la noticeInstitution
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School of Medicine Center for Biomedical Informatics and Genomics pays non établi dans la noticeUniversité ou école supérieure
Environmental and Occupational Health Sciences Institute, Connecticut Agricultural Experiment Station et Rutgers — The State University of New Jersey, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.