A Public Mid-Density Genotyping Platform for Lettuce ( Lactuca sativa L.)

Résumé fourni par la source

Small public breeding programs, particularly those focused on minor and specialty crops, often face significant limitations in resources dedicated to genetic research, breeding efforts, and the development of enabling technologies. The integration of molecular genetics and genomics into crop improvement requires the development, validation, and deployment of supporting tools. To support genetic studies and breeding efforts in lettuce ( Lactuca sativa L.), we developed the Lettuce 3K DArTag panel as a mid-density genotyping platform. Within the 3000 target marker loci distributed across the nine chromosomes are 41 markers associated with economically important traits and used for seedling selection. We validated the panel’s performance on a diverse set of accessions important for pre-breeding of native traits and for GWAS applications. We also confirmed the panel’s effectiveness in structured populations by constructing a linkage map from a biparental population. This genotyping resource offers a rapid, cost-effective solution for generating high-quality, genome-wide DNA markers applicable to trait discovery, diversity analysis, marker-assisted selection (MAS), genomic prediction, parental identification, varietal fingerprinting, and other applications supporting germplasm enhancement and varietal development in lettuce.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.