Induced Pluripotent Stem Cell-Derived Extracellular Vesicles Prevent Neural Stem Cell Senescence to Promote Cognitive Recovery after Traumatic Brain Injury
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Le résumé fourni par la source
Hippocampal neural stem cells (NSCs) have attracted significant attention due to their essential role in maintaining cognitive functions, such as memory and spatial orientation through neurogenesis. Cognitive impairment is a common and debilitating complication of traumatic brain injury (TBI), yet its underlying mechanisms remain poorly understood and effective clinical interventions are lacking. In this study, we observed persistent cognitive deficits in a mouse model of TBI, a phenomenon that has been widely documented in previous studies, and importantly, we found that these impairments were closely associated with increased hippocampal NSCs (H-NSCs) senescence. To investigate the cause of NSCs’ senescence, we analyzed cerebrospinal fluid samples from TBI patients and hippocampal tissues from TBI mice and identified persistently elevated levels of IL-1β post TBI. In vitro, IL-1β successfully induced NSCs’ senescence and suppressed neurogenesis. Induced pluripotent stem cell-derived small extracellular vesicles (iPSC-sEVs) reversed IL-1β-induced senescence and restored neurogenic potential in H-NSCs. In vivo, iPSC-sEVs alleviated cognitive deficits and H-NSC senescence after TBI. Integrated proteomic and NSC cell transcriptomic analyses revealed that the β-catenin/ID2/CDKN2B (p15 INK4b ) signaling axis plays a critical role in regulating H-NSC senescence, which was further validated through inhibitor experiments. In summary, our findings demonstrate that iPSC-sEVs attenuate NSC senescence and improve cognitive function following TBI via modulation of the β-catenin/ID2/CDKN2B (p15 INK4b ) axis.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Induced Pluripotent Stem Cell-Derived Extracellular Vesicles Prevent Neural Stem Cell Senescence to Promote Cognitive Recovery after Traumatic Brain Injury
- Date Crossref
- 23/10/2025
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Central South University Xiangya Hospital pays non établi dans la noticeUniversité ou école supérieure
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National Clinical Research pays non établi dans la noticeStructure de recherche
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Xiangya Hospital Central South University Department of Neurosurgery pays non établi dans la noticeÉtablissement de santé
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Tsinghua–Berkeley Shenzhen Institute pays non établi dans la noticeStructure de recherche
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Research Institute of Tsinghua University in Shenzhen pays non établi dans la noticeUniversité ou école supérieure
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Xiangya School of Medicine Department of Neurosurgery pays non établi dans la noticeUniversité ou école supérieure
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Research Institute of Brain and Spinal Cord Injury pays non établi dans la noticeStructure de recherche
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Bio-Intelligent Manufacturing and Living Matter Bioprinting Center pays non établi dans la noticeInstitution
Xiangya Hospital — Central South University, National Clinical Research et Department of Neurosurgery — Xiangya Hospital Central South University, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.