Investigation of the effect of chitosan-polycaprolactone scaffolds loaded with chrysin-capped silver nanoparticles in the healing of experimental cutaneous wound defects in rat

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Background and Aims: Nowadays, the use of antimicrobial agents with the lowest opportunity to develop resistance against microbes for the treatment of infections has been proposed as a new approach.This study aimed to use green silver nanoparticles (AgNPs) synthesized with chrysin as an antibacterial compound in wound healing. Materials and Methods:In this experimental study, chrysin was employed as a reducing agent for the synthesis of AgNPs.The synthesized nanoparticles were characterized for size, structural properties, and morphology.The nanoparticles were subsequently blended with a polycaprolactone-chitosan polymer mixture and electrospun into nanofibers.The antibacterial activity of these nanofibers was evaluated against Staphylococcus aureus.To assess the wound-healing properties of the nanofibers, full-thickness excisional wounds (1 cm in diameter) were created on rats and treated with the synthesized scaffolds.Skin samples were histopathologically examined to evaluate pathological characteristics.Results: The results showed that chrysin capped AgNPs with a diameter of about 101±15 nm were synthesized.Scaffolds containing AgNPs significantly inhibited the growth of S. aureus, compared to the scaffold and control groups.The in vivo results also showed that the rates of wound closure in the treatment and control groups were 85.7±10.05%and 69.4±3.8% on the 7 th day after treatment, respectively.These results showed that the use of chrysin capped AgNPs significantly reduced the size of the wound. Conclusion:The produced scaffolds can play an effective role in wound healing by reducing infection at the injury site.

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