Profiling of Epstein-Barr Virus microRNAs in Whole Blood and Exosomes in Multiple Sclerosis

Résumé fourni par la source

BACKGROUND: Multiple sclerosis (MS) is a neuroinflammatory, demyelinating disease with Epstein-Barr virus (EBV) suggested as a prerequisite for disease development. EBV expresses 44 microRNAs (miRNAs) with largely unknown functions, but they have been implicated in EBV-infected cell proliferation and immune evasion. OBJECTIVES: This study investigates EBV miRNAs levels in whole blood and plasma exosomes at baseline and after treatment, and in relation to disease activity based on age-adjusted NfL ratios, in 50 newly diagnosed patients with relapsing-remitting MS (RRMS). METHODS: in whole blood, measured by qPCR. Serum neurofilament light chain (NfL) and IL-6, plasma anti-EBNA1 antibody titers, occludin, and zonula occludens-1 were measured by various immunoassays. RESULTS: The miRNA profiling of whole blood revealed expression of BHRF1 miRNAs in most patients, indicating that EBV is in lytic phase or latency phase III. Higher levels of miR-BART14-3p were observed in patients classified with high disease activity. EBV miRNA levels correlated with anti-EBNA1 antibody titers, biomarkers of inflammation and tight junction proteins.

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