Activation of lands cycle-mediated inflammation in living macrophages exposed to label-free particulate matter

Le résumé fourni par la source

Particulate matter (PM) is a pollutant of great concern and has severe impacts on human health. Macrophages (Mφ) play a crucial role in the response to PM exposure, as they undergo metabolic reprogramming associated with inflammation. However, upon exposure to PM, the metabolic changes that occur in living Mφ remain unclear. Here, we present evidence that PM activates Lands cycle-mediated inflammatory mechanisms using 3D-holotomography (3D-HT) technique integrated with multi-omics analysis. We show that internalization of PM results in abnormal lipid droplet (LD) dynamics, potentially leading to changes in lipid metabolite levels. Additionally, our multi-omics data reveal that alterations in lipid metabolism mediated by the Lands cycle leads to the increased production of inflammatory lipid mediators, including lysophospholipids, ceramides and sphingomyelin. Furthermore, by performing lipid metabolic imaging in living Mφ, we elucidated changes in the activity of altered lipid metabolism and the spatiotemporal correlation between inflammation and LD dynamics. Our findings suggest that the developed approach can provide a new experimental strategy to identify lipid metabolism biomarkers in living cells upon PM exposure. • Advanced imaging analysis: 3D holotomography (3D-HT) enables real-time visualization of abnormal lipid droplet dynamics and lipid metabolic changes in live macrophages. • Multi-omics integration: Combining lipidomics and transcriptomics reveals key molecular pathways underlying PM-induced inflammation. • Lands cycle activation: PM exposure induces phospholipid remodeling, driving inflammation. • PM exposure triggers early inflammatory activation in macrophages, followed by LD accumulation.

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