A new external jugular venipuncture technique for efficient vascular access that exploits a murine anatomical variation

Résumé fourni par la source

Vascular access in mice is a cornerstone of biomedical research, with peripheral venous approaches like the lateral tail vein, retrobulbar venous sinus, facial vein, and saphenous vein being common. However, central venous approaches are challenging due to animal size and required expertise. To address this, we developed the Sternoclavicular joint-Targeted External jugular venipuncture Method (STEM). This technique provides reliable, longitudinal vascular access for frequent blood sampling using palpable surface anatomy landmarks. Moreover, STEM eliminates the need for fur shaving, specialized restraints, or deep sedation, allowing a single operator to perform the procedure safely and efficiently. Our protocol, based on a comprehensive anatomical analysis, revealed that the external jugular vein in mice traverses anteriorly to the clavicle before draining into the subclavian vein - a key anatomical difference from humans. This finding enabled a refined technique using the sternoclavicular joint as a landmark, improving the success and reproducibility of central venous access. Finally, STEM facilitates efficient blood collection and accurate intravenous administration with minimal setup time. It is straightforward and easily replicable, allowing researchers of all expertise levels to achieve high precision and reproducibility. The simplified learning process and consistent results make STEM valuable for various mouse-based experiments in biomedical research.

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