Self-On Aptasensor Based on Interface Oxidation-Induced H2O2 Etching Strategy for Ultrasensitive Detection of Myogenin

Le résumé fourni par la source

Rhabdomyosarcoma (RMS), a highly malignant tumor that is more common in children, has a high mortality rate. So far, there have been few reports on the early diagnosis of RMS. As an effective biomarker for RMS, the serum level of myoglobin (MyoG) is of significant value for the clinical diagnosis of RMS. In this work, an ultrasensitive electrochemiluminescence (ECL) self-on aptasensor was developed using the interface oxidation-induced H 2 O 2 etching strategy for trace detection of MyoG. Specifically, l -tryptophan ( l -Trp) and ascorbic acid reacted at a given voltage to produce H 2 O 2, which etched the external Ag shell of NaF 4:Eu 3+ @Ag and released the encapsulated NaF 4:Eu 3+ luminophores. Meanwhile, due to the high interfacial conductivity and catalytic activity of Au@Pt nanoblocks, the ECL emission of NaF 4:Eu 3+ was enhanced, achieving strong signal self-on. When the MyoG antigen was present, the MyoG aptamer- l -Trp (Apt- l -Trp) was separated from the electrode surface due to the specific binding between the MyoG antigen and Apt. Therefore, the generation of H 2 O 2 was interrupted, thus terminating the etching of the Ag shell and leading to signal off. Based on this, the proposed ECL aptasensor achieved sensitive detection of MyoG in a wide concentration range of 1 pg/mL to 10 μg/mL, with a detection limit of 296.15 fg/mL ( S/N = 3). The designed interface oxidation-induced H 2 O 2 etching strategy extends the construction means of self-on sensors and provides an effective reference for the diagnosis of early RMS.

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