Development and Clinical Evaluation of a Multiplexed Health Surveillance Panel Using Ultra High‐Throughput PRM‐MS in an Inflammatory Bowel Disease Cohort
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Le résumé fourni par la source
Abstract Despite advances in clinical proteomics, translating protein biomarker discoveries into clinical use remains challenging due to the technical complexity of the validation process. Targeted MS‐based proteomic approaches such as parallel reaction monitoring (PRM) offer sensitive and specific assays for biomarker translation. In this study, we developed a multiplex PRM assay using the Stellar mass spectrometry platform to quantify 57 plasma proteins, including 24 FDA‐approved biomarkers. Loading curves (11 points) were performed at 4 sample throughputs (100, 144, 180, and 300 samples per day) using independently optimized and scheduled PRM methods. Following optimization, an inflammatory bowel disease (IBD) cohort of plasma samples (493 IBD, 509 matched controls) was analyzed at a throughput of 180 samples per day. To monitor system performance, the study also included over 1000 additional injections for system suitability tests, low‐, middle‐, and high‐quality controls, washes, and blanks. Using this approach, we observed high quantifiability (linearity, sensitivity, and reproducibility) in the PRM assay and consistent data acquisition across a large cohort. We also validated the candidate IBD markers, C‐reactive protein and orosomucoid protein, identified in a recent discovery experiment.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Development and Clinical Evaluation of a Multiplexed Health Surveillance Panel Using Ultra High‐Throughput PRM‐MS in an Inflammatory Bowel Disease Cohort
- Date Crossref
- 21/09/2025
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Cedars-Sinai Medical Center pays non établi dans la noticeÉtablissement de santé
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Thermo Fisher Scientific (United States) pays non établi dans la noticeEntreprise
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Cedars‐Sinai Medical Center Los Angeles CA USA pays non établi dans la noticeÉtablissement de santé
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Both authors contributed equally to this work pays non établi dans la noticeInstitution
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Thermo Fisher Scientific San Jose CA USA pays non établi dans la noticeInstitution
Cedars-Sinai Medical Center, Thermo Fisher Scientific (United States) et Cedars‐Sinai Medical Center Los Angeles CA USA, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.