Distinct reduction in relative microglial glucose uptake compared to astrocytes and neurons upon isolation from the brain environment
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Le résumé fourni par la source
Introduction Microglial energy metabolism has gained attention for the treatment of neurodegenerative diseases. In vitro methods provide important insights; however, it remains unclear whether the metabolism of highly motile microglia is preserved outside their regular environment. Therefore, we directly compared the microglial glucose uptake in vivo and in vitro in mice. Methods Microglia and astrocytes were isolated from the brain using immunomagnetic cell sorting following [ 18 F]FDG injection in living mice, followed by gamma and single-cell radiotracing (scRadiotracing). Enriched cell fractions were incubated with excess [ 18 F]FDG (50,000-fold) in vivo , washed, and measured equivalently. For all fractions, radioactivity per cell was normalized to the injected or incubated radioactivity, and ratios of microglialuptake were calculated relative to astrocytes and the microglia/astrocyte-negative fraction. The experiment was repeated using a glucose-free buffer and validated by in vitro incubation without prior in vivo [ 18 F]FDG injection to exclude the influence of fasting and glucose injection. Results scRadiotracing results were compared against cell culture [ 18 F]-FDG incubation. The in vivo glucose uptake of microglia was higher when compared to astrocytes (50.4-fold, p < 0.0001) and non-microglia/ non-astrocyte cells (10.6-fold, p < 0.0001). Microglia still exhibited the highest glucose uptake in vitro, but with a distinct reduction in microglia-to-astrocyte (5.7-fold, p < 0.0015) and microglia-to-microglia/astrocyte-negative ratios (1.7 fold, p < 0.0001). Fasting and in vitro incubation were used to validate the results. Cell culture indicated low microglial uptake compared to that in neurons (1:100) or astrocytes (1:10). Discussion Compared to astrocytes and other cells, microglia show a distinct reduction in uptake in vitro compared to in vivo uptake. Our results emphasize that in vitro experiments should be interpreted with caution when studying microglial energy metabolism.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Distinct reduction in relative microglial glucose uptake compared to astrocytes and neurons upon isolation from the brain environment
- Date Crossref
- 15/09/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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LMU Klinikum pays non établi dans la noticeÉtablissement de santé
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Center for Clinical Research (United States) pays non établi dans la noticeEntreprise
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Ludwig-Maximilians-Universität München pays non établi dans la noticeUniversité ou école supérieure
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Munich Cluster for Systems Neurology pays non établi dans la noticeStructure de recherche
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Johns Hopkins University pays non établi dans la noticeUniversité ou école supérieure
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German Cancer Research Center Partner Site Munich pays non établi dans la noticeStructure de recherche
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Heidelberg University pays non établi dans la noticeUniversité ou école supérieure
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German Center for Neurodegenerative Diseases pays non établi dans la noticeStructure de recherche
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LMU University Hospital Department of Nuclear Medicine pays non établi dans la noticeUniversité ou école supérieure
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Section of Clinical and Comparative Neuropathology pays non établi dans la noticeÉtablissement de santé
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University of Munich Munich Cluster for Systems Neurology (SyNergy) pays non établi dans la noticeUniversité ou école supérieure
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Johns Hopkins School of Medicine Russell H. Morgan Department of Radiology and Radiological Sciences pays non établi dans la noticeUniversité ou école supérieure
LMU Klinikum, Center for Clinical Research (United States) et Ludwig-Maximilians-Universität München, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.