Cigarette Smoke-Induced Cell Morphology via Keyence and Zeiss Microscopy

Le résumé fourni par la source

Cigarette smoke exposure induces oxidative stress, disrupts gene regulation, and causes morphological alterations in lung epithelial cells.While conventional methods such as qPCR and Western blotting are often used to study these effects, they are costly, labor-intensive, and unsuitable for rapid screening.In this study, we introduce a cost-effective imaging strategy to characterize early cellular responses of human bronchial epithelial cells (HBEpCs) to cigarette smoke extract (CSE).For the first time, the Keyence VHX-7000 digital microscope was employed to systematically analyze CSE-induced morphological changes, with direct comparison to the established Zeiss Axiovert 200M inverted fluorescence microscope.Quantitative analysis revealed consistent trends across both systems, demonstrating that the Keyence platform effectively captures morphological alterations with high throughput and minimal sample preparation.These findings establish the Keyence VHX-7000 as a viable, scalable alternative for preliminary cellular injury assessments, offering a practical tool for accelerating research into smoking-related lung and cardiovascular diseases.

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