Duck hepatitis A virus 3CD and 3D proteins are key facilitators of hnRNP K expression and cytoplasmic entry, and hijack hnRNP K to facilitate viral translation and replication
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Le résumé fourni par la source
Picornaviruses, constituting a diverse family of viral pathogens, cause numerous important diseases in humans and animals. The pathogenesis of these viruses is associated with many host factors. Studying host‒virus interactions is crucial for obtaining an in-depth understanding of viral pathogenesis. Here, we found that the picornavirus duck hepatitis A virus type 1 (DHAV-1) induces the expression of cellular heterogeneous nuclear ribonucleoprotein K (hnRNP K) and facilitates its nucleocytoplasmic shuttling to interact with DHAV-1 genomic RNA. hnRNP K positively regulates DHAV-1 translation and replication. hnRNP K utilizes its nucleic acid binding activity to interact directly with the DHAV-1 IRES and 3'UTR. The finding of this interaction between hnRNP K and the viral 3'UTR is important because it has not been reported in previous studies on RNA viruses. Furthermore, screening of DHAV-1 proteins identified the nonstructural proteins 3CD and 3D as key facilitators of hnRNP K expression and cytoplasmic entry. Meanwhile, hnRNP K interacts with 3CD and 3D through its KH1 and KI domains, with the KI domain being the most critical. 3CD and 3D also bind to DHAV-1 untranslated regions. These interactions help hnRNP K facilitate DHAV-1 translation and replication. In summary, hnRNP K forms complexes with 3CD and 3D, which binds to untranslated regions, promoting DHAV-1 translation and replication. These findings reveal a novel host‒virus interaction mechanism supporting viral translation and replication, offering new insights into picornavirus pathogenesis.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Duck hepatitis A virus 3CD and 3D proteins are key facilitators of hnRNP K expression and cytoplasmic entry, and hijack hnRNP K to facilitate viral translation and replication
- Date Crossref
- 01/11/2025
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Sichuan Center for Disease Control and Prevention pays non établi dans la noticeOrganisme public
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Guizhou University pays non établi dans la noticeUniversité ou école supérieure
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Yangzhou University pays non établi dans la noticeUniversité ou école supérieure
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Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education of the People's Republic of China pays non établi dans la noticeStructure de recherche
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Veterinary Department in College of Animal Science Institute of Veterinary Immunology and Green Drugs pays non établi dans la noticeUniversité ou école supérieure
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Ltd. Sinopharm Yangzhou VAC Biological Engineering Co. pays non établi dans la noticeEntreprise
Sichuan Center for Disease Control and Prevention, Guizhou University et Yangzhou University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.