High-Resolution Quantitative Reconstruction of Microvascular Architectures in Mouse Hepatocellular Carcinoma Models
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Le résumé fourni par la source
Background/Objectives: Alterations in liver vascularization play a remarkable role in liver disease development, including hepatocellular carcinoma (HCC), but remain understudied. This study evaluated the hepatic microvascular imaging method and provided high-resolution quantitative anatomical data on the characteristics and architecture of liver vasculature in wild-type (WT) mice and HCC mouse models. Methods: C57BL/6 mice were injected with Akt/Ras or Sleeping Beauty transposon to induce HCC. Liver tissues from normal and Akt/Ras mice underwent hematoxylin and eosin, Masson’s trichrome, Ki67, and lymphatic endothelial receptor-1 staining. Using cutting-edge high-definition fluorescence micro-optical sectioning tomography, high-precision microvascular visualization of the liver was performed in WT and Akt/Ras HCC mice. Results: The sectioned volumes of normal and HCC liver tissues were 204.8 mm3 and 212.8 mm3, respectively. The microvascular systems associated with the tissues of the Akt/Ras HCC mouse were twisted, disordered, and compressed by tumor nodules. In the four tumor nodules, the path of the hepatic artery was more around the tumor edge, whereas the portal vein occupied the central position and constituted the main blood vessel entering the tumors. The porosity of HCC and paracancerous cirrhotic tissues was significantly less than that of normal tissues. The radii of the central vessels in the hepatic sinusoid of paratumoral cirrhotic tissues were significantly higher than those of normal tissues; however, the hepatic sinusoid density of paratumoral cirrhotic tissues was lower. Conclusions: This research provides a deeper understanding of the normal liver microvasculature and alterations in cases of cirrhosis and HCC, which complements scientific insights into liver morphology and physiology. This straightforward research approach involving the novel 3D liver microvasculature can be used in multiscale physiological and pathophysiological studies regarding liver diseases.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- High-Resolution Quantitative Reconstruction of Microvascular Architectures in Mouse Hepatocellular Carcinoma Models
- Date Crossref
- 14/08/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Medical Center Hospital pays non établi dans la noticeÉtablissement de santé
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Xi’an International University pays non établi dans la noticeUniversité ou école supérieure
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State Key Laboratory of Chemical Engineering pays non établi dans la noticeStructure de recherche
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Tsinghua University Department of Chemical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Xijing Hospital pays non établi dans la noticeÉtablissement de santé
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Air Force Medical University pays non établi dans la noticeUniversité ou école supérieure
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Northwest University Department of Liver Diseases and Interventional Radiology pays non établi dans la noticeUniversité ou école supérieure
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State Key Laboratory for Manufacturing Systems Engineering pays non établi dans la noticeStructure de recherche
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Fourth Military Medical University Department of Nuclear Medicine pays non établi dans la noticeUniversité ou école supérieure
Medical Center Hospital, Xi’an International University et State Key Laboratory of Chemical Engineering, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.