Rapid Multiplex Detection of Viral Pathogens in Whole Blood Using Microfluidic Sample Processor and Smartphone-Linked Handheld Instrument

Le résumé fourni par la source

An integrated system for rapid multiplex of whole blood samples for simultaneous detection of Zika, Dengue (serotypes 1 and 3), and Chikungunya is demonstrated using a smartphone assisted detection device and microfluidic sample processor. The microfluidic processor stores all required reagents for sample lysis as well as LAMP master mix and partitions the final solution into 6 compartments for detection of 4 virus assays plus two experimental controls. With a handheld detection instrument that provides uniform 65°C heating, LED illumination, and a smartphone camera for image gathering, the multiplex assay is detected through LAMP-generated fluorescence within the distinct compartments. This system achieves detection limits of live virus spiked into whole blood through the ranges of 2×105- 6×106PFU/ml and lacks cross reactivity between assays while providing a pipette free operation with no additional equipment.

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