Aptamer-functionalized magnetic covalent organic frameworks for rapid extraction and LC-MS/MS quantification of gentamicin in milk

Résumé fourni par la source

Gentamicin (GEN) residues in food products, arising from its extensive use in veterinary medicine, have prompted stringent regulations and a demand for simple, rapid detection methods. In this study, aptamer-modified magnetic covalent organic frameworks (COFs), denoted as TpDBD@Fe₃O₄@apt, were synthesized and employed as adsorbents in magnetic solid-phase extraction (MSPE) for the highly specific capture and enrichment of GEN residues in milk. Several factors influencing MSPE efficiency were systematically evaluated and optimized. Coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS), the developed method exhibited good linearity within the linear range (R 2 ≥ 0.999), low detection limits (0.48–0.54 ng/mL), satisfactory recoveries (92.7 %–108.8 %), and high precision (RSDs ≤10.8 %). The proposed method was successfully applied to real milk samples, providing an efficient solution for the detection of GEN residues.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.