Positive effects of single-nanosized oxygen nanobubble water on exercise tolerance: A randomized crossover trial

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The effect of single-nanosized oxygen nanobubble water (NBO 2 W), an oxygen-enriched beverage, on exercise performance remains unclear. This double-blind, randomized, placebo-controlled crossover trial investigated whether NBO 2 W intake influences lactate threshold (LT), a key indicator of aerobic exercise performance. The participants (n = 20; age, 47.4 ± 5.0 years; female, 12) consumed either NBO 2 W or placebo water (PW) immediately followed by an incremental exercise test on an ergometer for symptom-limited exhaustion. During the test, the heart rate, respiratory gas analysis (VO 2 and VCO 2 ), tissue oxygen index (TOI), and blood lactate level were continuously monitored. The first administered beverage was randomly selected, with both participants and investigators blinded to the beverages. A washout period of minimum seven days was implemented between the two tests. The mean peak VO 2 was 26.13 ± 5.05 mL/min/kg. Eighteen (90 %) participants exercised less than four times per week, indicating limited exercise habits. The average washout period was 9.7 ± 5.0 days. The work rate (WR) at LT was significantly higher after NBO 2 W intake than after PW intake (mean difference = 3.33 W, p = 0.03). The WR was not significantly different at peak VO 2 or at the onset of blood lactate accumulation. Furthermore, NBO 2 W intake significantly increased the lactate levels 5 min post-exercise (mean difference = 0.88, p = 0.01) and decreased the TOI (mean difference = −3.8, p = 0.01). Short-term NBO 2 W consumption in three doses, including that on the day before exercise, did not affect the peak exercise capacity, but improved the WR at LT. • This trial investigated whether NBO 2 W intake influences lactate threshold (LT). • The mean peak VO 2 was 26.13 ± 5.05 mL/min/kg. • WR did not significantly differ at peak VO 2 or onset of blood lactate accumulation. • Three-dose NBO 2 W did not affect peak exercise capacity, but improved WR at LT.

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