A pyroelectric-based approach for high-sensitive detection of stress-related biomarkers

Le résumé fourni par la source

The ability to print biomolecules is crucial in biomedical research, drug discovery, biosensors, and diagnostics, enabling detailed molecular studies. Structural changes in biomolecules like Tau, α-synuclein, or αβ 1-42 amyloid play a key role in neurodegenerative diseases, making them targets for early diagnosis and astronaut health monitoring. A novel technique, pyro-electrohydrodynamic jetting (p-jet), was introduced. This study validates p-jet’s effectiveness in concentrating diluted biomolecules into fluorescent microscale spots. Despite progress, optimizing binding strategies for low-abundance proteins remains challenging. We assessed BSA immobilization in p-jet spots, confirming its reliability for immunodetection. Various immobilization strategies were evaluated. We report the first detection of pre-aggregated Tau in artificial urine at 130 fM. With minimal sample needs and low cost, p-jet offers a promising tool for neurodegeneration diagnosis and point-of-care use.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.