Single‐Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa ( Medicago sativa L.)
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Le résumé fourni par la source
Anthers consist of various specialised cell types and play a significant role in plant reproduction. Although the molecular mechanisms underlying anther development and regulation have been extensively studied, the single-cell transcriptional landscape and dynamic regulation during anther development in M. sativa remain largely unexplored. In the present study, we constructed the first single-cell transcriptome atlas of M. sativa anthers to provide a comprehensive view of cell type-specific gene expression and epigenetic modifications. The reconstruction of the developmental trajectories of tapetum and microspores led to the identification of novel genes and elucidated the regulatory networks involved in tapetum formation. Our findings revealed rapid functional transitions in tapetum during the tetrad stage, including cell formation, specialisation, and programmed cell death (PCD). Additionally, we analysed the stages at which tapetal degradation and microspore shrinkage occurred in the sterile line. Overall, this study offers valuable insights into the molecular mechanisms underlying anther development in M. sativa. Specifically, MsKIN14P was identified as a key regulator of microtubules and the cytoskeleton during mitosis, and the transcription factors ERF3 and ERF025 were shown to influence anther development through the ethylene response pathways. These findings provide an essential theoretical foundation for the development of novel male-sterile lines and enhance the breeding capabilities of M. sativa.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Single‐Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa ( <i>Medicago sativa</i> L.)
- Date Crossref
- 29/07/2025
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Jilin Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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Jilin Provincial Academy of Forestry Science pays non établi dans la noticeInstitution
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Jilin Academy of Agricultural Sciences pays non établi dans la noticeStructure de recherche
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Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding College of Forestry and Grassland Science Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding pays non établi dans la noticeUniversité ou école supérieure
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Institute of Agricultural Biotechnology pays non établi dans la noticeStructure de recherche
Jilin Agricultural University, Jilin Provincial Academy of Forestry Science et Jilin Academy of Agricultural Sciences, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.