INVESTIGATING THE PRESENCE OF MYCOBACTERIA IN THE WATER PIPES CONSUMED IN CAFES AND TEA GARDENS

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ABSTRACT: Objectives; It’s aimed to investigate the presence of Mycobacteria in the water pipes consumed in cafes and tea gardens in Hatay province. Methods; After determining the cafes and tea gardens which water pipes are consumed in, 250 samples were taken from water pipes. After samples were decontaminated and homogenised, they were stained with the method of Erlich Ziehl Neelsen (EZN) and were examined for acid-fast bacilli (ARB). For cultures of the samples, Lowenstein Jensen (L-J) media (BioMerieux, France) and BacT-ALERT MP (BioMerieux, France) were used. The nitrat reduction, niacin accumulation and catalase tests were made for identification of the strains isolated. Results; In our study, in 9 (3,6%) of 250 samples ARB were detected. Nontuberculous mycobacteria (NTM) were isolated from 27 (10,8%) samples by L-J media and from 75 (30%) samples by automated system. There was growth in both L-J as well as automated system in 24 (9,6%) samples. Thereby while isolating NTM in 78 (31,2%) of 250 samples, in none of them M. tuberculosis complex was isolated. Conclusions; It was thought that NTM strains might be in the tap water added into the water pipes or might be contaminated by infected users. Water pipe consumption found objectionable because of isolation of NTM in our study as NTM strains increasingly cause illness in recent years besides the many agents of infectious diseases especially M. tuberculosis.

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