Detection and Molecular Characterization of Rift Valley Fever Virus in Apparently Healthy Cattle in Uganda
Rattachement africain : Ouganda, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Rift Valley fever (RVF) is a zoonotic disease caused by the Rift Valley fever virus (RVFV), affecting humans, livestock, and wild ruminants. This study aimed to characterize and assess the genetic diversity of RVFV strains circulating among livestock in Uganda. Blood samples were collected between January 2021 and May 2024 from apparently healthy cattle, goats, and sheep in four districts. The samples were first screened for RVFV antibodies using ELISA; antibody-positive samples were subsequently tested for viral RNA using reverse transcriptase quantitative PCR (RT-qPCR). The PCR-positive samples underwent targeted amplicon sequencing, and phylogenetic analyses of the small (S) and large (L) genome segments were conducted to determine viral lineages. Of the 833 ELISA-positive samples, 10 (all from cattle) tested positive for RVFV RNA using RT-qPCR. Consensus sequences were successfully generated for six S segments and one L genome segment. A phylogenetic analysis revealed that all sequences belonged to lineage C, showing close genetic similarity to RVFV strains previously identified in Uganda, Kenya, Sudan, Madagascar, and Saudi Arabia. Limited genetic diversity was observed at both the nucleotide and amino acid levels. The detection of RVFV in apparently healthy cattle suggests ongoing, low-level viral circulation in Uganda. These findings offer important insights for guiding RVF surveillance, control, and policymaking in the country.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Detection and Molecular Characterization of Rift Valley Fever Virus in Apparently Healthy Cattle in Uganda
- Date Crossref
- 20/07/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Ministry of Agriculture Ouganda (code pays fourni par la source)Organisme public
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Uganda National Health Research Organisation Ouganda (code pays fourni par la source)Organisation à but non lucratif
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Animal and Plant Health Inspection Service pays non établi dans la noticeOrganisme public
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Makerere University Makerere University, Ouganda (code pays fourni par la source)Université ou école supérieure
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National Animal Disease Diagnostics and Epidemiology Centre (NADDEC) Entebbe P.O. Box, Ouganda (pays nommé en fin d’affiliation)Institution
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Foreign Animal Disease Diagnostic Laboratory pays non établi dans la noticeStructure de recherche
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College of Veterinary Medicine Makerere University, Ouganda (pays nommé en fin d’affiliation)Université ou école supérieure
Ministry of Agriculture (Ouganda), Uganda National Health Research Organisation (Ouganda) et Animal and Plant Health Inspection Service, avec 4 autres affiliations. Pays d’affiliation : Ouganda.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.