Backbone Protecting Groups for Enhanced Peptide and Protein Synthesis
Rattachement africain : au, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Solid‐phase peptide synthesis has become an indispensable technique for the routine preparation of linear peptides of up to approximately 40 amino acids in length. However, the solid‐phase approach is still hampered by chain insolubility and aggregation, which reduces synthetic yields. Moreover, many of the deletion impurities that can form are often chromatographically inseparable from the target sequence, which diminishes final product purity. The use of backbone N ‐protecting groups can ameliorate this synthetic inefficiency by increasing peptide chain solubility and suppressing aggregation. Backbone protection is also useful for promoting peptide macrocyclization, suppressing common side reactions in peptide chemistry, and improving solution‐phase handling. Commercially available precursors containing benzyl‐based groups and pseudoprolines have found widespread use, in academic laboratories and industry. A range of other strategies have also been investigated in a bid to increase the utility of backbone protecting groups and to develop more efficient methods for their introduction and removal. This review provides a comprehensive account of the state of the art, and includes detailed synthetic methods relating to the use of backbone protection, and its application to “difficult” peptides and proteins of biological significance. The strengths and weaknesses of each approach are analyzed, and a commentary on future directions is provided.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Backbone Protecting Groups for Enhanced Peptide and Protein Synthesis
- Date Crossref
- 16/07/2025
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The University of Melbourne pays non établi dans la noticeUniversité ou école supérieure
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PolyPeptide Group (United States) pays non établi dans la noticeEntreprise
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Florey Institute of Neuroscience and Mental Health pays non établi dans la noticeStructure de recherche
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Molecular Research Institute pays non établi dans la noticeStructure de recherche
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University of Melbourne School of Chemistry School of Chemistry AUSTRALIA pays non établi dans la noticeUniversité ou école supérieure
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PolyPeptide Laboratories (Sweden) AB Limhamnsvägen 108 pays non établi dans la noticeInstitution
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PolyPeptide Group QC and Development SWEDEN pays non établi dans la noticeInstitution
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The Florey 30 Royal Parade Parkville Victoria 3052 Australia pays non établi dans la noticeInstitution
The University of Melbourne, PolyPeptide Group (United States) et Florey Institute of Neuroscience and Mental Health, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.