LncRNA NEAT1 promotes epithelial–mesenchymal transition in nasal polyp cells via the miR-199-3p/PAK4 axis
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Le résumé fourni par la source
Background and purpose Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent inflammatory condition marked by high recurrence and limited therapeutic efficacy. This study investigates the role of long non-coding RNA NEAT1 in promoting epithelial–mesenchymal transition (EMT) in CRSwNP, focusing on its regulatory interaction with the miR-199-3p/PAK4 axis. Methods NEAT1 expression was assessed in nasal epithelial cells from CRSwNP patients using qPCR and FISH. Primary human nasal epithelial cells and BEAS-2B cells were subjected to NEAT1 knockdown via siRNA. Cell migration, barrier function, and cytoskeletal dynamics were evaluated through scratch assays, Transwell migration, FITC-Dextran permeability testing, and phalloidin staining. EMT marker expression was analyzed via Western blotting and immunofluorescence. Transcriptome sequencing identified PAK4 as a downstream effector. In vivo validation was performed using a mouse nasal polyp model, and molecular interactions among NEAT1, miR-199-3p, and PAK4 were confirmed via dual-luciferase reporter assays. Rescue experiments further elucidated mechanistic pathways. Results In comparison to controls, NEAT1 expression was significantly elevated in the epithelial tissues of CRSwNP. NEAT1 knockdown inhibited cell migration, enhanced epithelial barrier integrity, and reversed EMT-associated cytoskeletal remodeling. E-cadherin levels increased, while N-cadherin and vimentin decreased. Transcriptomic and functional analyses identified PAK4 as a NEAT1-regulated target. NEAT1 was shown to sponge miR-199-3p, thereby relieving its inhibitory effect on PAK4. Overexpression of miR-199-3p suppressed PAK4 and mitigated EMT-related changes induced by NEAT1. Conclusion NEAT1 promotes EMT in nasal polyp epithelial cells by modulating the miR-199-3p/PAK4 axis, highlighting its potential as a diagnostic biomarker and therapeutic target in CRSwNP.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- LncRNA NEAT1 promotes epithelial–mesenchymal transition in nasal polyp cells via the miR-199-3p/PAK4 axis
- Date Crossref
- 30/06/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The Affiliated Yongchuan Hospital of Chongqing Medical University pays non établi dans la noticeÉtablissement de santé
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Chongqing Medical University Department of Otorhinolaryngology pays non établi dans la noticeUniversité ou école supérieure
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First People's Hospital of Chongqing pays non établi dans la noticeÉtablissement de santé
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Chongqing Cancer Hospital pays non établi dans la noticeÉtablissement de santé
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Chongqing Hospital of Traditional Chinese Medicine Department of Pediatrics pays non établi dans la noticeÉtablissement de santé
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Chongqing University Cancer Hospital Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment pays non établi dans la noticeUniversité ou école supérieure
The Affiliated Yongchuan Hospital of Chongqing Medical University, Department of Otorhinolaryngology — Chongqing Medical University et First People's Hospital of Chongqing, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.