eRNA and eDNA co-extraction from water samples filtered through 47mm diameter filters (NucleoMag DNA/RNA Water Kit - MACHEREY NAGEL). v1

Le résumé fourni par la source

The objective of this protocol is the environmental RNA (eRNA) and DNA (eDNA) co-extraction from water samples filtered through 47 mm diameter filters. RNA extraction is performed using a MagnetaPure 32 Nucleic Acid Purification System (Dutscher) and with the NucleoMagDNA/RNA Water Kit (Macherey Nagel), with a rDNase digestion step. DNA extraction is performed using a MagnetaPure 32 Nucleic Acid Purification System (Dutscher) and with the NucleoMagDNA/RNA Water Kit (Macherey Nagel). The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. The benefits of using the MagnetaPure 32 Nucleic Acid Purification System (Dutscher) are increased productivity and repeatability, as well as eliminating human error and the pain of repetitive work. This protocol is used prior to molecular biology analysis (e.g. qPCR, metabarcoding, ddPCR) to specifically target both macro- and micro-organisms eRNA and eDNA extracted from water samples. This protocol is optimised for rare eDNA/RNA and the suggested elution volumes are therefore low (between 50 and 65 µL), but can be increased if targets are more abundant.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.