Simultaneous determination of five active compounds in compound Salvia Miltiorrhiza gel via multi-channel HPLC detection

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ABSTRACT Objective To establish a high-performance liquid chromatographic method (HPLC) for simultaneous determination of two phenolic acids (gallic acid, salvianolic acid B) and three quinones (cryptotanshinone, tanshinone I, tanshinone IIA) in Compound Salvia miltiorrhiza Gel (CSG). Methods The HPLC method employs gradient elution with multi-channel to optimize detection sensitivity. Validation parameters include linearity, precision, stability, repeatability, and accuracy. Results All five compounds exhibit excellent linearity (R 2 >0.999) within their respective concentration ranges.The method demonstrates high precision (RSD < 2%), stability (RSD < 1.93%), repeatability (RSD < 1.90%), and accuracy (average recoveries: 98.93–101.31%). No interference is observed in negative control samples. Conclusion This validated HPLC method provides a robust and efficient approach for quality control of CSG, ensuring accurate quantification of its key bioactive components. The study supports the standardization of herbal gel formulations and offers a foundation for further pharmacological research.

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